Jul 14, 2025
15
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Biotechnology(or biotech) is the use of biological systems found in organisms or the use of the living organisms themselves to make technological advances and adapt those technologies to various fields. Biotechnology uses living organisms or their parts to develop technologies in medicine, agriculture, industry, food processing, biofuels, and the environment. Advances like genetic engineering, recombinant DNA, PCR, and CRISPR underpin its modern age. In biotechnology in medicine and health, it delivers diagnostics, therapeutics (e.g., gene therapy, vaccines, insulin) via engineered microbes. In biotechnology in agriculture, it enables GM crops—stress‑resistant, biofortified varieties-to boost yield and food security. Industrial biotech (white biotech) drives biotechnology in industries and environmental solutions (bioremediation), while biotechnology in food processing and biofuels support bio‑economy growth-India’s biotech sector grew from US $10 billion in 2014 to US $165.7 billion by 2024.
Biotechnology is broadly defined as the application of biological systems and living organisms to develop products or processes for specific purposes. This includes ancient techniques like fermenting grapes into wine or milk into yogurt, as well as cutting-edge methods like gene editing. The term comes from Greek “bio” (life) and “technology” (skill).
In essence, biotech allows scientists to manipulate genes and cells to achieve desired outcomes. For instance, recombinant DNA technology (inserting genes from one organism into another) has enabled production of human insulin in bacteria. Likewise, techniques like PCR (Polymerase Chain Reaction) amplify DNA quickly for diagnostics. Biotechnology is crucial for solving problems in healthcare, agriculture, industry, and environmental conservation. For UPSC exams, topics like GMOs, gene therapy, and biotech innovations are frequently highlighted, underscoring the importance of grasping this foundational concept.
Table of content

Red Biotechnology (Medicine & Healthcare) – Focuses on healthcare applications: vaccines, gene therapy (e.g. CAR‑T), insulin, therapeutic proteins. Vital for biotechnology in medicine and biotechnology in healthcare.
Green Biotechnology (Agriculture & Animal Healthcare) – Enhances crops and livestock: GM crops (Bt cotton, Golden Rice), biofertilizers, disease-resistant animals. Core to biotechnology in agriculture and animal healthcare.
White Biotechnology (Industry & Food) – Industrial-scale fermentation: enzymes, biofuels, bioplastics, food production like cheese and beer. Important for biotechnology in industries and biotechnology in food.
Blue Biotechnology (Marine/Freshwater) – Explores marine life for pharmaceuticals, cosmetics, bio-oils/algae biofuels.
Yellow Biotechnology (Food & Insects) – Enhances food via fermentation and insect biotech for pest control or novel ingredients.
Grey Biotechnology (Environmental) – Bioremediation, waste-to-energy, pollution control, ecosystem conservation.
Brown Biotechnology (Arid-zone) – Develops biotech solutions for desert and dryland agriculture.
Purple (Violet) Biotechnology (Ethics & IP) – Covers legal, ethical, and patent aspects of biotech.
Gold Biotechnology (Bioinformatics) – Computational biology, genomics, proteomics, big data in biotech R&D.
Black/Dark Biotechnology (Biowarfare) – Concerns defense and misuse of biotech in bioterrorism.
The remarkable progress in biotechnology has been driven by advanced laboratory techniques that enable precise manipulation, replication, and analysis of biological material. Below are key biotechnological methods revolutionizing the field:
Genetic Engineering
Genetic engineering involves the modification of an organism’s DNA using lab-based techniques to introduce desirable traits or produce specific proteins.
Recombinant DNA Technology (RDT):
Gene Identification & Isolation: Uses restriction endonucleases to cut the gene of interest from the source DNA.
Vector Insertion: The same enzymes cut matching sites on vectors (like plasmids) for insertion.
Gene Cloning & Expression: The recombinant plasmid is introduced into a host (commonly bacteria), which is cultured to express the inserted gene by producing proteins.
Polymerase Chain Reaction (PCR):
In Vitro DNA Amplification: Replicates DNA segments outside living cells.
Primer Binding: Synthetic primers target specific genome segments.
DNA Amplification: Taq polymerase enzyme rapidly multiplies the segment through cycles.
Application: Widely used for detecting infectious diseases through rapid DNA amplification.
RNA Interference (RNAi)
RNAi is a natural gene-silencing mechanism in eukaryotic organisms, where small RNA fragments bind to mRNA and prevent protein translation.
Targets and suppresses specific gene expressions.
Used to:
Study gene function and pathways.
Knock down harmful genes in diseases.
Develop pest-resistant plant varieties.
Tissue Culture
Tissue culture is the artificial cultivation of tissues or cells (plant or animal) in controlled laboratory conditions.
Plant Tissue Culture:
Utilizes the totipotency of plant cells to regenerate whole plants from tissue fragments.
Allows propagation and genetic enhancement of economically important plants.
Applications:
Research on plant growth, metabolism, reproduction, and nutrient needs.
Diagnostic tools for detecting infections, enzyme deficiencies, and chromosomal abnormalities.
Development of drugs, vaccines, and cancer classification systems.
Bioinformatics
Bioinformatics is an interdisciplinary science combining biology, mathematics, and computer science to manage and analyze complex biological data.
Develops software and algorithms for processing biological datasets.
Supports large-scale experiments like genome sequencing and gene expression profiling.
Applications:
Drug discovery and design.
Genomic studies and personalized medicine.
Functional analysis of genes and proteins.
CRISPR-CAS9
CRISPR‑Cas9 is a groundbreaking biotechnology tool adapted from a bacterial defense system that “remembers” viruses by storing viral DNA snippets.
Guide RNA directs the Cas9 enzyme to cut specific DNA sequences, enabling precise genome editing.
It’s revolutionizing biotechnology in medicine and biotechnology in healthcare, with research targeting single-gene disorders like cystic fibrosis, hemophilia, and sickle cell disease.
In biotechnology in agriculture, it's being used to engineer disease-resistant crops and improve yields.
Broader applications include biotechnology in industries (e.g., fermentation) and driving biotechnology UPSC‑relevant innovations.
Biotechnology’s applications span many fields. Below are key applications, each vital for understanding biotech’s role in society and for UPSC preparation:
Biotechnology in Nutrient Supplementation
Biofortified foods: Biotechnology enables infusion of essential nutrients into staple crops to combat malnutrition. A prime example is Golden Rice, engineered to produce beta-carotene (Vitamin A). Golden Rice helps prevent Vitamin A deficiency in populations reliant on rice.
Enhanced nutrition: Similar approaches create iron-rich or protein-enriched cereals. Such nutrient supplementation in food aid and diet is a powerful biotech tool to improve public health.
Biotechnology for Abiotic Stress Resistance
Stress-tolerant crops: Biotech develops plant varieties that can withstand harsh conditions (drought, salinity, heat) on limited arable land. For example, genetic engineering can introduce drought-resistant traits into crops grown in arid regions. This is critical as only ~20% of the earth’s land is highly fertile. Enhancing salt or frost tolerance helps ensure stable food production under climate stress.
Food security: Such crops allow farming on marginal lands and help feed growing populations with fewer resources. E.g., in drought-prone areas of Africa, biotech tools have produced crop varieties that survive dry spells, increasing yields per hectare.
Biotechnology in Industries
Bioprocessing: The use of enzymes and microbes in manufacturing – called industrial or white biotech – is widespread. Examples include using yeast to brew alcohol, bacteria to produce bioplastics, or enzymes in detergents.
Bio-based products: Industrial biotech replaces chemical processes with biological ones for sustainability. For instance, biocatalysts can produce polymers like acrylamide with less pollution than conventional chemistry.
Economy: This sector covers everything from biofuels to enzyme production for food, pharmaceuticals, and textiles.
Biotechnology in Strengthening Fibres
Spider silk in goats: One high-profile biotech innovation is producing spider silk proteins in goat milk. Spider silk is extremely strong and lightweight; goats have been engineered to express spider silk genes in their milk.
Advanced materials: The collected silk proteins can be spun into fibers stronger than steel by weight, with uses in bulletproof vests and medical sutures. This approach is easier than farming spiders and shows how biotech can create next-generation materials.
Biotechnology in Biofuels
Renewable fuels: Biotechnology enables production of bioethanol and biodiesel from crops (corn, sugarcane) and waste. For example, fermenting corn produces ethanol fuel. These biofuels burn cleaner than fossil fuels, reducing greenhouse gas emissions.
Future of energy: As oil supplies tighten, biotech-driven biofuels (even cellulosic ethanol from grass stalks) are key alternatives to power vehicles and industry without carbon pollution.
Biotechnology in Healthcare
Therapeutic proteins: Biotech produces vital medicines. For example, human insulin is now produced by genetically engineered bacteria rather than animal pancreases. Other monoclonal antibodies and enzymes (for diabetes, cancer, etc.) are made with biotech methods.
Vaccines and gene therapy: Modern vaccines (including some COVID-19 vaccines) use genetic techniques to elicit immunity. Biotech also enables gene therapy – inserting healthy genes to treat genetic disorders. These breakthroughs transform patient care.
Diagnostics: Techniques like PCR and ELISA (bioassays) are biotechnological tools for rapid disease diagnosis. For instance, PCR can detect HIV infection or trace genetic mutations early.
Animals Biotechnology
Created by inserting foreign DNA—are a cornerstone of biotechnology, especially in biotechnology in medicine and healthcare, with over 95% being mice used for research on gene regulation, development, and disease.
In biotechnology in agriculture and animal healthcare, livestock and fish are engineered for traits like disease resistance, enhanced milk or wool yield, exemplified by “Rosie” the cow producing human α‑lactalbumin milk.
Biotechnology in industries taps transgenics for chemical/toxicity testing and safety screening, including more sensitive toxicity and vaccine assays like polio using Tg‑PVR mice.
In biotechnology in food, transgenic animals deliver biological products—e.g., therapeutic proteins in milk—to lower production costs and improve nutrition.
Biotechnology in Food Processing
Fermentation: Traditional and industrial fermentation use microbes to convert raw materials into food with longer shelf life. Examples include yogurt, cheese, beer and bread. Biotechnology improves these processes, ensuring safety and efficiency.
Value addition: Biotech processes (like yeast fermentation) add flavors, nutrients or preserve foods, making inedible or perishable foods into stable consumables.
Biotechnology: Fuel from Waste
Waste-to-energy: Bioremediation uses microbes to convert organic waste (plant waste, sewage, industrial effluents) into biofuels (methane, ethanol). For example, certain bacteria digest paper mill waste and release methane usable as fuel.
Sustainable recycling: This approach not only generates energy but also manages waste with fewer pollutants, turning garbage into a resource.
Biotechnology in Commodity Chemicals and Specialty Chemicals
Green chemistry: Microbial biocatalysts can produce bulk chemicals (e.g. bio-acetone) without harsh chemicals. Using enzymes requires less energy and produces fewer toxic byproducts.
Biodegradable plastics: Biotechnology enables creation of bio-based polymers (like polylactic acid) for eco-friendly plastics, reducing dependence on petrochemicals.
Biotechnology in Hi-Tech Finishing Fabrics
Improved textiles: Biotechnology enhances fabrics. For instance, biotech cotton is engineered to be stronger, more absorbent, and wrinkle-resistant.
Bio-processing: Enzymes called bio-polishing enzymes are used in fabric finishing to improve dye uptake and softness. Such fabrics dry quicker and hold colors better.
Biotechnology in Detergent Proteases
Enzymatic cleaners: Many modern detergents contain proteases and other enzymes from bacteria to break down protein and starch stains.
Eco-friendly detergents: These enzymes reduce the need for harsh chemicals. After washing, enzyme-containing biomass left in laundry can even be used as organic fertilizer.
Biotechnology in Wound Dressings
Biopolymers: Biotechnology has led to medical dressings coated with chitosan, a biopolymer from shrimp and crab shells.
Healing and protection: Chitosan dressings accelerate wound healing and are biodegradable. They exemplify how biotech materials can improve healthcare products.
While biotech offers great promise, its application raises significant concerns. Aspirants should be aware of these issues:
Ethical concerns: Altering genes can provoke moral debates (e.g. GM animals, designer babies). Patenting genetic sequences has raised ethical questions about owning life forms.
Environmental impact: Unintended effects on ecosystems may occur if GMOs spread beyond intended fields. For example, Bt toxins in soil could affect non-target insects. Biodiversity and wild species can be impacted by engineered organisms.
Health and safety risks: Long-term effects of GMO foods or new gene therapies are not fully known. There are debates on allergenicity or genetic resistance due to consumption of GM products.
Socio-economic issues: Biotech can widen inequalities. Patents and ownership can concentrate benefits in large corporations, potentially limiting farmers’ access to seeds.
Biocontainment and biosecurity: Advanced biotech like synthetic biology could be misused (e.g. bioweapons). Strict biosecurity and regulation are needed to prevent malicious use.
Regulatory challenges: Establishing safety standards and public trust is complex. Balancing innovation with regulation (e.g. for CRISPR gene editing) is an ongoing challenge.
These challenges underscore that biotech must be pursued with caution, transparency, and robust governance.
India’s Bio-economy Surge: Grew from US $10 billion in 2014 to US $165.7 billion by 2024, contributing 4.25% of GDP with a CAGR of 17.9%—on track for a $300 billion target by 2030 under the Bio‑E3 (“Economy, Employment, Environment”) policy.
National Biopharma Mission (NBM): Launched in 2017 with ₹1,500 Cr (US $250 million WB co‑funding); has supported 100+ projects, 30 MSMEs, and helped launch ~19 products (e.g. DNA COVID‑19 vaccine, liraglutide biosimilar)
Also implemented Innovate in India (i3) for entrepreneurship, tech transfer offices, and shared infrastructure.
Plans underway for NBM 2.0 to develop 6–10 novel biopharma products over 5 years.
BIRAC & BIG Scheme: Under DBT, BIRAC has nurtured startups (from ~50 to ~11,000 by mid‑2025) via funds like AcE, Biofoundries, Bio‑AI hubs, and the BIG grant scheme.
BioSaarthi Mentorship: Launched March 2025 to connect biotech startups with global mentors for scale‑up and export readiness
Sectoral Missions:
Biotech KISAN (2017): Empowers biotechnology in agriculture and animal healthcare by linking labs with farmers .
Atal Jai Anusandhan Biotech Mission: Focuses on maternal-child health, AMR, vaccines, nutrition, and clean tech platforms.
One Health Consortium (2021): Integrates human, livestock, wildlife, and environmental disease surveillance .
Infrastructure & Industry Growth:
DBT-established Biotech Parks & Incubators support SMEs in biotechnology in industries, food, and healthcare.
Collaboration with Miltenyi Biotec India (June 2025) strengthens capabilities in cell & gene therapy
State-level Boost: Odisha approved a ₹1,113 Cr biotechnology development scheme (2025–2030) to leverage regional biodiversity for medical, agri, and environmental biotech
To harness biotechnology’s benefits while mitigating risks, experts suggest:
Strong regulations and oversight: Develop clear biosafety laws and ethical guidelines for research and products.
Public engagement: Educate and involve society in biotech debates to build trust and informed consent.
Research investment: Promote sustainable R&D focusing on green and safe technologies. Governments and institutions should fund public-sector biotech research.
Collaborations: Encourage partnerships between academia, industry, and farming communities (e.g. India’s Biotech KISAN programme) to translate innovation to the field.
Global cooperation: Share technology and best practices internationally to address global issues (food security, pandemics). Strengthened biosecurity measures and cooperation can help prevent misuse of biotech.
With proactive policies (like India’s National Biopharma Mission, BIRAC, and biotech parks) and a focus on sustainability, biotechnology can drive economic growth and social development.
Q.What is biotechnology(or biotech)?
A.Biotechnology is the use of living organisms, cells, or biological processes to develop products and technologies. It merges life sciences with engineering to create applications in healthcare, agriculture, and industry.
Q.How is biotechnology used in agriculture?
A.In agriculture, biotech is used to create GM crops with desirable traits. Examples include Bt cotton (pest-resistant cotton) and Golden Rice (rich in Vitamin A). These crops can yield more food, resist pests or stress, and improve nutrition for farmers and consumers.
Q.What are the benefits of biotechnology?
A.Biotechnology benefits society by producing medicines (like insulin and vaccines) safely at large scale, enhancing food security through higher crop yields and nutrition, and creating eco-friendly industrial processes (e.g. biofuels, biodegradable plastics). It also enables breakthroughs like personalized medicine and sustainable waste management.
Q.What are the challenges of biotechnology?
A.Major challenges include ethical concerns (e.g. genetic modifications, patents on life), environmental risks (potential impact on biodiversity), safety issues (unknown long-term effects of GM foods or therapies), and the need for strong regulations to ensure biotech is used safely and equitably.
Biotechnology is a transformative field for 21st-century challenges. Its applications—from healthcare (life-saving drugs, gene therapies) to agriculture (GM crops boosting yield and nutrition) and industry (green manufacturing, biofuels)—offer solutions that can improve lives worldwide. For UPSC aspirants, understanding biotech concepts (like recombinant DNA, GMOs, bioethics) is essential for both the science and current affairs portions of the exam.
However, biotechnology also raises important ethical, environmental and socio-economic questions that require balanced policies and public discourse. For example, Golden Rice and Bt cotton illustrate both the potential benefits (reduced malnutrition, fewer pesticides) and the controversies (biosafety, corporate control) of biotech in agriculture.
Looking ahead, technological advances (like CRISPR gene editing and personalized medicine) promise even greater impact. Through careful regulation, investment in research, and international cooperation, biotechnology can continue to advance India’s bio-economy and meet global needs sustainably. For UPSC candidates, a deep grasp of biotechnology’s applications and challenges will not only aid exam preparation but also highlight its relevance to India’s development.
Internal Linking Suggestions
How to Begin Your UPSC Preparation : The Ultimate Guide For Beginners
UPSC Previous Year Question Papers with Answers PDF - Prelims & Mains (2014-2024)
upsc optional subject list and syllabus-for-cse-exam-2025-complete-guide
How to Prepare Current Affairs for UPSC Exam: A Comprehensive Guide
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External Linking Suggestions
UPSC Official Website – Syllabus & Notification: https://upsc.gov.in/
Press Information Bureau – Government Announcements: https://pib.gov.in/
NCERT Official Website – Standard Books for UPSC: https://ncert.nic.in